SLC30A2 Monoclonal antibody

SLC30A2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D9E5

Cat no : 67993-1-Ig

Synonyms

FLJ36708, PP12488, SLC30A2, Zinc transporter 2, ZnT 2, ZNT2

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Validation Data Gallery

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  WB analysis using 67993-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67993-1-Ig (SLC30A2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67993-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67993-1-Ig (SLC30A2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67993-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67993-1-Ig (SLC30A2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HaCaT cells, Caco-2 cells, HT-29 cells, HeLa cells, NCCIT cells, JAR cells, ATDC-5 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67993-1-Ig targets SLC30A2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC30A2 fusion protein Ag8232
• Full Name: solute carrier family 30 (zinc transporter), member 2
• Calculated Molecular Weight: 323 aa, 35 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC006251
• Gene Symbol: SLC30A2
• Gene ID (NCBI): 7780
• RRID: AB_2918742
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC30A2, also named as the Zn transporter 2 gene (ZnT2), belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. It plays a role in the zinc ion transmembrane transport. Expression of SLC30A2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, Paneth cells, and mammary epithelial cells (MECs). SLC30A2 consists of six transmembrane domains with cytoplasmic N- and C-termini that contain numerous regulatory domains, and functions as a homo- or hetero- dimer to transport Zn into vesicles (PMID: 29476070 ). SLC30A2 has 2 isoforms with the molecular mass of 35 and 41 kDa.