SLC2A9 Monoclonal antibody

SLC2A9 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig and More (3)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

2D6E1

Cat no : 67530-1-Ig

Synonyms

Glucose transporter type 9, GLUT 9, GLUT9, GLUTX, SLC2A9, UAQTL2, URATv1

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Validation Data Gallery

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  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67530-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67530-1-Ig (SLC2A9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67530-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67530-1-Ig (SLC2A9 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human hepatocirrhosis using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human hepatocirrhosis tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human kidney using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human hepatocirrhosis using 67530-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human hepatocirrhosis tissue using SLC2A9 antibody (67530-1-Ig, Clone: 2D6E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: SMMC-7721 cells, pig liver tissue, HepG2 cells, L02 cells, HuH-7 cells, rat liver tissue, mouse liver tissue, rabbit liver tissue, HSC-T6 cells
• Positive IHC detected in: human liver tissue, human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human hepatocirrhosis tissue, human kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

67530-1-Ig targets SLC2A9 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Rabbit, Pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SLC2A9 fusion protein Ag24135
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 9
• Calculated Molecular Weight: 540 aa, 59 kDa
• Observed Molecular Weight: 56-59 kDa
• GenBank Accession Number: BC018897
• Gene Symbol: SLC2A9
• Gene ID (NCBI): 56606
• RRID: AB_2882749
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Metabolites; Anti-Hyperuricemic Effects of Extracts from Chaenomeles speciosa (Sweet) Nakai Fruits on Hyperuricemic Rats; Authors - Ruoling Xu
rat	IHC	Evid Based Complement Alternat Med; Effects of a Macroporous Resin Extract of Dendrobium officinale Leaves in Rats with Hyperuricemia Induced by Anthropomorphic Unhealthy Lifestyle; Authors - Lin-Zi Li
mouse	WB	J Pharm Pharmacol; Antihyperuricemic and nephroprotective effects of extracts from Orthosiphon stamineus in hyperuricemic mice.; Authors - Wen-Hao Xu
mouse	WB	Curr Res Food Sci; Effects of polysaccharides-riched Prunus mume fruit juice concentrate on uric acid excretion and gut microbiota in mice with adenine-induced chronic kidney disease; Authors - Yan Huang
rat	IF	Front Pharmacol; Ameliorative action of "daitongxiao" against hyperuricemia includes the "uric acid transporter group"; Authors - Lijie Zheng
human,mouse	WB	Biomed Pharmacother; Sanghuangporus vaninii ethanol extract alleviates hyperuricemic renal injury by regulating the uric acid transporters and inhibiting HK-2 apoptosis; Authors - Liping Hua