GLUT3 Polyclonal antibody

• Featured Product
• KD/KO Validated

GLUT3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

68

Reviews (2)

Cat no : 20403-1-AP

Synonyms

GLUT 3, SLC2A3, GLUT-3

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Validation Data Gallery

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  WB analysis using 20403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 20403-1-AP

  U-251 cells were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20403-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20403-1-AP (GLUT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20403-1-AP (GLUT3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20403-1-AP (GLUT3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20403-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20403-1-AP (GLUT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Caco-2 using 20403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using GLUT3 antibody (20403-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of Caco-2 using 20403-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using GLUT3 antibody (20403-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, U-251 cells, Jurkat cells, HeLa cells, C6 cells, mouse brain tissue, rat brain tissue, HepG2 cells
• Positive IHC detected in: mouse testis tissue, human lung cancer tissue, human breast cancer tissue, human placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Caco-2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 56 publications below
• IHC: See 12 publications below
• IF: See 16 publications below
• IP: See 1 publications below

Product Information

20403-1-AP targets GLUT3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GLUT3 fusion protein Ag14203
• Full Name: solute carrier family 2 (facilitated glucose transporter), member 3
• Calculated Molecular Weight: 496 aa, 54 kDa
• Observed Molecular Weight: 48-60 kDa
• GenBank Accession Number: BC039196
• Gene Symbol: GLUT3
• Gene ID (NCBI): 6515
• RRID: AB_10694437
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glucose transporter 3 (GLUT3), also known as solute carrier family 2, facilitated glucose transporter member 3 (SLC2A3), is a transporter protein regulating glucose transport across cell membranes and is a primary glucose transporter in neurons.

What is the molecular weight of GLUT3? Is GLUT3 post-translationally modified?

The molecular weight of GLUT3 transporter is 49-52 kDa and depends on the glycosylation pattern, which is tissue-specific (PMID: 9253355 and 9889355). GLUT3 can be N-glycosylated.

What is the subcellular localization of GLUT3?

Glucose transporters, including GLUT3, are multiple-pass integral membrane proteins. GLUT3 is present at the plasma membrane but is also a subject of recycling between the plasma membrane and endosomes. 

What molecules can be transported by GLUT3?

Although the main substrate of GLUT3 transport is glucose, it can also transport mannose, galactose, and xylose.

What is the tissue expression pattern of GLUT3?

GLUT1 and GLUT3 are important for the transport of glucose into the central nervous system. While GLUT1 transports glucose through blood vessels and into astrocytes, GLUT3 is predominantly expressed in neurons, being their main glucose transporter (PMID: 9302083), and in testis (PMID: 18577699).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	IF	Neuron; Sympathetic nerve-enteroendocrine L cell communication modulates GLP-1 release, brain glucose utilization, and cognitive function; Authors - Wenran Ren
human	WB	Mol Cancer; CircEZH2/miR-133b/IGF2BP2 aggravates colorectal cancer progression via enhancing the stability of m6A-modified CREB1 mRNA.; Authors - Bing Yao
human	WB	Cell Death Differ; A viral interferon regulatory factor degrades RNA-binding protein hnRNP Q1 to enhance aerobic glycolysis via recruiting E3 ubiquitin ligase KLHL3 and decaying GDPD1 mRNA.; Authors - Xiaoyu Qi
human	WB	Autophagy; Newcastle disease virus degrades SIRT3 via PINK1-PRKN-dependent mitophagy to reprogram energy metabolism in infected cells.; Authors - Yabin Gong
human	WB	Cell Death Dis; The ubiquitin-like protein UBTD1 promotes colorectal cancer progression by stabilizing c-Myc to upregulate glycolysis; Authors - Liqin Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

balawant (Verified Customer) (12-25-2023)

This antibody is very specific and has no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1200
• Cell Tissue Type: Renal Cancer cell line caki-1 and caki-2

FH

Hua (Verified Customer) (02-03-2023)

This antibody works ok. There is one strong non-specific band around 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2, mouse liver, and mouse brain tissues

GLUT3 Antibody Western Blot validation (1:1000 dilution) in HepG2, mouse liver, and mouse brain tissues (Cat no:20403-1-AP)

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