SLC26A11 Polyclonal antibody

SLC26A11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14156-1-AP

Synonyms

Sodium-independent sulfate anion transporter, kidney brain anion transporter, KBAT

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Validation Data Gallery

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  WB analysis using 14156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14156-1-AP (SLC26A11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 14156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14156-1-AP (SLC26A11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 14156-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 14156-1-AP (SLC26A11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, unboiled mouse brain tissue, mouse kidney tissue, unboiled mouse kidney tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14156-1-AP targets SLC26A11 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC26A11 fusion protein Ag5338
• Full Name: solute carrier family 26, member 11
• Calculated Molecular Weight: 606 aa, 65 kDa
• Observed Molecular Weight: 65-72 kDa
• GenBank Accession Number: BC035900
• Gene Symbol: SLC26A11
• Gene ID (NCBI): 284129
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC26A11 is a sodium-independent sulfate transporter from high endothelial venules (PMID: 12626430). SLC26A11 exhibits sodium-independent sulfate anion transporter activity that may cooperate with SLC26A2 to mediate DIDS-sensitive sulfate uptake into high endothelial venules endothelial cells (HEVEC) (PMID: 12626430). SLC26A11 is critical for inhibitory transmission and contributes to locomotor coordination in Purkinje cells (PMID: 27390771). SLC26A11, a chloride transporter, localizes with the vacuolar H+-ATPase of A-intercalated cells of the kidney and can be detected at 72kDa (PMID: 21716257).