SLC25A6-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC25A6-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 14841-1-AP

Synonyms

SLC25A6, ANT 3, ANT 2, ADP/ATP translocase 3, N-terminally processed, ADP/ATP translocase 3

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Validation Data Gallery

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  WB analysis using 14841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14841-1-AP

  WB result of SLC25A6-Specific antibody (14841-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A6-Specific transfected HEK-293 cells.

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  WB analysis of human liver using 14841-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14841-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14841-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14841-1-AP

  IP result of anti-SLC25A6-Specific (IP:14841-1-AP, 4ug; Detection:14841-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human skeletal muscle using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skeletal muscle using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver using 14841-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14841-1-AP (SLC25A6-Specific Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HepG2 using 14841-1-AP

  Immunofluorescent analysis of HepG2 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

• 

  IF Staining of MCF-7 using 14841-1-AP

  Immunofluorescent analysis of MCF-7 cells using 14841-1-AP (SLC25A6-Specific antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: L02 cells, HEK-293 cells, human liver tissue, human heart tissue, human placenta tissue, HEK-293T cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human skeletal muscle tissue, human heart tissue, human kidney tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Product Information

14841-1-AP targets SLC25A6-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 6
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: NM_001636
• Gene Symbol: SLC25A6
• Gene ID (NCBI): 293
• RRID: AB_2190371
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Curr Biol; Defining the Substrate Spectrum of the TIM22 Complex Identifies Pyruvate Carrier Subunits as Unconventional Cargos.; Authors - Ridhima Gomkale
human	WB,CoIP	Am J Cancer Res; PTPMT1 regulates mitochondrial death through the SLC25A6-NDUFS2 axis in pancreatic cancer cells; Authors - Peng-Peng Ding
	WB	Hum Mol Genet; Mutations of the mitochondrial carrier translocase channel subunit TIM22 cause early-onset mitochondrial myopathy.; Authors - David Pacheu-Grau
human	WB,IF	Cancer Cell Int; Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer.; Authors - Yi Hao
human	WB	FEBS Lett; TIM29 is a subunit of the human carrier translocase required for protein transport.; Authors - Sylvie Callegari
human	WB,IHC,CoIP	iScience; MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells; Authors - Xingzhao Ji