ANT1/2 Polyclonal antibody
ANT1/2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 17796-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | K-562 cells, HepG2 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat liver tissue |
Positive IHC detected in | human liver tissue, human liver cancer tissue, mouse heart tissue, mouse kidney tissue, human cervical cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 8 publications below |
IHC | See 5 publications below |
Product Information
17796-1-AP targets ANT1/2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ANT1/2 fusion protein Ag11956 |
Full Name | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 |
Calculated Molecular Weight | 298 aa, 33 kDa |
Observed Molecular Weight | 30-32 kDa, 60 kDa |
GenBank Accession Number | BC056160 |
Gene Symbol | SLC25A5 |
Gene ID (NCBI) | 292 |
RRID | AB_2190358 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)
Protocols
Product Specific Protocols | |
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WB protocol for ANT1/2 antibody 17796-1-AP | Download protocol |
IHC protocol for ANT1/2 antibody 17796-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Elife Legionella pneumophila modulates host energy metabolism by ADP-ribosylation of ADP/ATP translocases. | ||
Mol Cell Proteomics Proteomics of skin proteins in psoriasis: from discovery and verification in a mouse model to confirmation in humans. | ||
Exp Neurol Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder. | ||
J Proteome Res Proteomic Identification of the SLC25A46 Interactome in Transgenic Mice Expressing SLC25A46-FLAG. | ||
Biology (Basel) TERT Promoter Revertant Mutation Inhibits Melanoma Growth through Intrinsic Apoptosis. |