SLC25A10 Polyclonal antibody

• Featured Product
• KD/KO Validated

SLC25A10 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12086-1-AP

Synonyms

DIC, SLC25A10

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Validation Data Gallery

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  WB analysis using 12086-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12086-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 12086-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12086-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12086-1-AP (SLC25A10 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12086-1-AP

  IP result of anti-SLC25A10 (IP:12086-1-AP, 3ug; Detection:12086-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human lung using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 12086-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12086-1-AP (SLC25A10 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, mouse liver tissue, mouse kidney tissue, HepG2 cells, PC-3 cells
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lung tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

12086-1-AP targets SLC25A10 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC25A10 fusion protein Ag2719
• Full Name: solute carrier family 25 (mitochondrial carrier; dicarboxylate transporter), member 10
• Calculated Molecular Weight: 296 aa, 32 kDa
• Observed Molecular Weight: 29-31 kDa
• GenBank Accession Number: BC015797
• Gene Symbol: SLC25A10
• Gene ID (NCBI): 1468
• RRID: AB_10859816
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A10, also known as dicarboxylate ion carrier (DIC), is a mitochondrial inner membrane carrier protein which catalyzes the transport of dicarboxylates such as malate and succinate across the mitochondrial membrane in exchange for phosphate, sulfate, and thiosulfate. SLC25A10 is expressed at highest levels in kidney and liver, lower levels in lung, spleen, heart, and brain, and lowest levels in skeletal muscle. Two isoforms of SLC25A10 exist due to the alternative splicing.

Publications

Species	Application	Title
human	IHC,WB	Hum Cell; The role of PYCR1 in inhibiting 5-fluorouracil-induced ferroptosis and apoptosis through SLC25A10 in colorectal cancer; Authors - Borong Zhou
human	WB,IHC	Oncol Lett; SLC25A10 performs an oncogenic role in human osteosarcoma.; Authors - Gaoyuan Wang ; KD Validated
human,rat	WB,IF,IHC	J Cardiovasc Transl Res; Mild Therapeutic Hypothermia Alleviated Myocardial Ischemia/Reperfusion Injury via Targeting SLC25A10 to Suppress Mitochondrial Apoptosis; Authors - Senlin Ma