SLC25A1 Recombinant antibody

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  WB analysis using 84696-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84696-1-RR (SLC25A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 84696-1-RR

  WB result of SLC25A1 antibody (84696-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A1 transfected HeLa cells.

• Featured Product
• KD/KO Validated

SLC25A1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84696-1-RR

Clone No.241931G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Tricarboxylate carrier protein, SLC20A3, CTP, Citrate transport protein, CIC

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, NCI-H1299 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84696-1-RR targets SLC25A1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC004980
• Gene Symbol: SLC25A1
• Gene ID (NCBI): 6576
• RRID: AB_3672112
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P53007
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 have been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. (PMID: 24681808, 23561848)