SLC22A7 Polyclonal antibody

SLC22A7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 26796-1-AP

Synonyms

hOAT2, NLT, Novel liver transporter, OAT2, Organic anion transporter 2, SLC22A7

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Validation Data Gallery

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  WB analysis of mouse kidney using 26796-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26796-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 26796-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 26796-1-AP

  IP result of anti-SLC22A7 (IP:26796-1-AP, 4ug; Detection:26796-1-AP 1:1000) with mouse liver tissue lysate 5200ug.

• 

  IHC staining of human liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

The immunogen of 26796-1-AP is SLC22A7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC22A7 fusion protein Ag25217
• Full Name: solute carrier family 22 (organic anion transporter), member 7
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC033805
• Gene Symbol: SLC22A7
• Gene ID (NCBI): 10864
• RRID: AB_2880639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; Upregulation of histone acetylation reverses organic anion transporter 2 repression and enhances 5-fluorouracil sensitivity in hepatocellular carcinoma.; Authors - Yingying Wang
mouse	WB	Nutrients; Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.; Authors - Karolin Weitkunat
human	WB	Drug Metab Pharmacokinet; Inhibiting uptake activity of organic anion transporter 2 by bile acids.; Authors - Yu Wang
mouse	WB	Xenobiotica; Changes in the expression of drug-metabolising enzymes and drug transporters in mice with collagen antibody-induced arthritis; Authors - Chieri Fujino
mouse		Am J Physiol Renal Physiol; Tubular Dysfunction Impairs Renal Excretion of Pseudouridine in Diabetic Kidney Disease; Authors - Anna V Mathew