SLC22A7 Polyclonal antibody

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  WB analysis of mouse kidney using 26796-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 26796-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 26796-1-AP (SLC22A7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 26796-1-AP

  IP result of anti-SLC22A7 (IP:26796-1-AP, 4ug; Detection:26796-1-AP 1:1000) with mouse liver tissue lysate 5200ug.

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  IHC staining of human liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 26796-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26796-1-AP (SLC22A7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC22A7 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 26796-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

OAT2, NLT, hOAT2

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

26796-1-AP targets SLC22A7 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC22A7 fusion protein Ag25217
• Full Name: solute carrier family 22 (organic anion transporter), member 7
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC033805
• Gene Symbol: SLC22A7
• Gene ID (NCBI): 10864
• RRID: AB_2880639
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y694
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Organic anion transporter (OAT)2 (SLC22A7) belongs to the solute carrier group of membrane transport proteins that mediate cellular uptake of numerous organic ions including xenobiotics and endogenous substrates (PMID: 9529348, 20190416). SLC22A7 was originally identified as a novel liver-specific transporter because of its predominant mRNA expression in the rat liver (PMID: 15900017, 25904762). Human SLC22A7 exhibits a robust transport function for a wide array of naturally occurring nucleobases, nucleosides, and nucleotides with a particular role for cGMP (PMID: 18216183).

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; Upregulation of histone acetylation reverses organic anion transporter 2 repression and enhances 5-fluorouracil sensitivity in hepatocellular carcinoma.; Authors - Yingying Wang
mouse	WB	Nutrients; Effect of Microbial Status on Hepatic Odd-Chain Fatty Acids Is Diet-Dependent.; Authors - Karolin Weitkunat
human	WB	Drug Metab Pharmacokinet; Inhibiting uptake activity of organic anion transporter 2 by bile acids.; Authors - Yu Wang
mouse	WB	Xenobiotica; Changes in the expression of drug-metabolising enzymes and drug transporters in mice with collagen antibody-induced arthritis; Authors - Chieri Fujino
mouse		Am J Physiol Renal Physiol; Tubular Dysfunction Impairs Renal Excretion of Pseudouridine in Diabetic Kidney Disease; Authors - Anna V Mathew