OCTN2 Polyclonal antibody

• 

  WB analysis of mouse small intestine using 16331-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 16331-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16331-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 16331-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16331-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16331-1-AP (OCTN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 16331-1-AP

  IP result of anti-OCTN2 (IP:16331-1-AP, 4ug; Detection:16331-1-AP 1:500) with mouse skeletal muscle tissue lysate 2200ug.

• 

  IHC staining of human kidney using 16331-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16331-1-AP (OCTN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16331-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16331-1-AP (OCTN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

OCTN2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16331-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

SLC22A5, Solute carrier family 22 member 5, High-affinity sodium-dependent carnitine cotransporter

---

Tested Applications

• Positive WB detected in: mouse small intestine tissue, mouse skeletal muscle tissue, human placenta tissue, mouse pancreas tissue, mouse testis tissue
• Positive IP detected in: mouse skeletal muscle tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below

Product Information

16331-1-AP targets OCTN2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OCTN2 fusion protein Ag9450
• Full Name: solute carrier family 22 (organic cation/carnitine transporter), member 5
• Calculated Molecular Weight: 557 aa, 63 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC012325
• Gene Symbol: OCTN2
• Gene ID (NCBI): 6584
• RRID: AB_2191406
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O76082
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OCTN2 (Organic Cation/Carnitine Transporter 2), encoded by SLC22A5, is a ubiquitously expressed organic anion/cation transporter. OCTN2 plays a key role in absorption, tissue distribution and renal reabsorption of L-carnitine which is an essential cofactor in fat metabolism. Defects in the OCTN2 are responsible for primary carnitine deficiency. OCTN2 also transports some important respiratory medicines (such as tiotropium), and due to its expression in the lung, may influence the disposition and absorption of these medicines in the lung.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.; Authors - Judith Simcox
rat	WB	Food Funct; Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota; Authors - Nanhai Zhang
mouse	WB	Nutrients; The Enhancement of Acylcarnitine Metabolism by 5-Heptadecylresorcinol in Brown Adipose Tissue Contributes to Improving Glucose and Lipid Levels in Aging Male Mice; Authors - Kuiliang Zhang
rat	WB	Front Pharmacol; L-carnitine prevents lenvatinib-induced muscle toxicity without impairment of the anti-angiogenic efficacy; Authors - Zheng Jing
human	WB	J Neurochem; Identification of drug transporters contributing to oxaliplatin-induced peripheral neuropathy.; Authors - S Fujita ; KD Validated
rat	WB	Toxicol Lett; Lenvatinib causes reduced expression of carnitine/organic cation transporter 2 and carnitine deficiency in the skeletal muscle of rats.; Authors - Zheng Jing