SLC22A4 Recombinant antibody

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  WB analysis using 83192-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83192-1-RR (SLC22A4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83192-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83192-1-RR against Human SLC22A4 were performed. The affinity constant is 1.08 nM.

SLC22A4 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83192-1-RR

Clone No.240064D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

UT2H, OCTN1, ETTh, ETT, 240064D5

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83192-1-RR targets SLC22A4 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SLC22A4 fusion protein Ag3974
• Full Name: solute carrier family 22 (organic cation/ergothioneine transporter), member 4
• Calculated Molecular Weight: 551 aa, 62 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: BC028313
• Gene Symbol: SLC22A4
• Gene ID (NCBI): 6583
• RRID: AB_3670882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H015
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC22A4 (solute carrier family 22 member 4), also known as OCTN1. It is expected to be located in cell membrane and mitochondrion membrane. It is strongly expressed in kidney, trachea, bone marrow and fetal liver and in several human cancer cell lines, but not in adult liver (PMID: 9426230). The protein functions as a Na+-dependent and pH-dependent high affinity microbial symporter of potent food-derived antioxidant ergothioeine (PubMed:15795384), which transports one sodium ion with one ergothioeine molecule.