SLC22A4 Polyclonal antibody

SLC22A4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13238-1-AP

Synonyms

Ergothioneine transporter, ET transporter, ETT, OCTN1, SLC22A4, UT2H

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Validation Data Gallery

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  WB analysis using 13238-1-AP

  boiled and 37°C incubated mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 13238-1-AP (SLC22A4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse kidney tissue, 37°C incubated mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13238-1-AP targets SLC22A4 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC22A4 fusion protein Ag3974
• Full Name: solute carrier family 22 (organic cation/ergothioneine transporter), member 4
• Calculated Molecular Weight: 551 aa, 62 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC028313
• Gene Symbol: SLC22A4
• Gene ID (NCBI): 6583
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC22A4 (solute carrier family 22 member 4), also known as OCTN1. It is expected to be located in cell membrane and mitochondrion membrane. It is strongly expressed in kidney, trachea, bone marrow and fetal liver and in several human cancer cell lines, but not in adult liver (PMID: 9426230). The protein functions as a Na+-dependent and pH-dependent high affinity microbial symporter of potent food-derived antioxidant ergothioeine (PubMed:15795384), which transports one sodium ion with one ergothioeine molecule. The calculated molecular weight of SLC22A4 is 62 kDa, the band of 70 kDa is glycosylated (PMID:26724204).