SLC20A1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SLC20A1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12423-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis of HEK-293 using 12423-1-AP (same clone as 12423-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 12423-1-AP (same clone as 12423-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human kidney using 12423-1-AP (same clone as 12423-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12423-1-AP (same clone as 12423-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 12423-1-AP (same clone as 12423-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 12423-1-AP (same clone as 12423-1-PBS)

  IP result of anti-SLC20A1 (IP:12423-1-AP, 4ug; Detection:12423-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 12423-1-AP (same clone as 12423-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12423-1-PBS in a different storage buffer formulation.

---

Product Information

12423-1-PBS targets SLC20A1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC20A1 fusion protein Ag3084
• Full Name: solute carrier family 20 (phosphate transporter), member 1
• Calculated Molecular Weight: 679 aa, 74 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC019944
• Gene Symbol: SLC20A1
• Gene ID (NCBI): 6574
• RRID: AB_2286212
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SLC20A1 (also known as PiT1) is a high-affinity sodium-dependent phosphate (Pi) import protein. It is widely expressed and plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions. SLC20A1 is involved in cell proliferation, tumor growth and cell signaling independently of its transport function. SLC20A1 also functions as a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.