EAAT4 Polyclonal antibody

EAAT4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12876-1-AP

Synonyms

EAAT4, SLC1A6

---

Validation Data Gallery

• 

  WB analysis of A375 using 12876-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12876-1-AP (EAAT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12876-1-AP targets EAAT4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EAAT4 fusion protein Ag3554
• Full Name: solute carrier family 1 (high affinity aspartate/glutamate transporter), member 6
• Calculated Molecular Weight: 61.6 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC028721
• Gene Symbol: EAAT4
• Gene ID (NCBI): 6511
• RRID: AB_10642147
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Excitatory amino acid transporter 4 (EAAT4) is a neuronal glutamate transporter responsible for the reuptake of synaptically released glutamate. The EAAT4 protein is highly enriched in Purkinje cells of the cerebellum, although it is not restricted to these cells (18770868). While the predicted molecular weight of EAAT4 is 62 kDa, considerable heterogeneity in transporter size has been reported in the brain with molecular weights ranging from 50 to 120 kDa as a result of differential glycosylation and/or phosphorylation (24056007). This antibody recognizes 60-70 kDa of EAAT4 in mouse brain and several other lysate.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Impact of Reduced Cerebellar EAAT Expression on Purkinje Cell Firing Pattern of NPC1-deficient Mice.; Authors - Michael Rabenstein