SLC19A3 Polyclonal antibody

SLC19A3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Publications

10

Reviews (2)

Cat no : 13407-1-AP

Synonyms

SLC19A3, Thiamine transporter 2, ThTr 2, THTR2

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Validation Data Gallery

• 

  WB analysis using 13407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13407-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13407-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13407-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13407-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13407-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13407-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13407-1-AP

  IP result of anti-SLC19A3 (IP:13407-1-AP, 4ug; Detection:13407-1-AP 1:600) with mouse liver tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue, SH-SY5Y cells, mouse skeletal muscle tissue, mouse kidney tissue, human placenta tissue, rat heart tissue, 3T3-L1 cells, rat liver tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IF: See 1 publications below

Product Information

13407-1-AP targets SLC19A3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC19A3 fusion protein Ag4053
• Full Name: solute carrier family 19, member 3
• Calculated Molecular Weight: 496 aa, 56 kDa
• Observed Molecular Weight: 63-70 kDa
• GenBank Accession Number: BC032014
• Gene Symbol: SLC19A3
• Gene ID (NCBI): 80704
• RRID: AB_2187997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Sci Transl Med; CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.; Authors - Sara Picó
human	WB	Nat Commun; Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia; Authors - Alaullah Sheikh
human	WB	Front Cell Dev Biol; Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.; Authors - Jane L Tarry-Adkins
human	WB,IF	J Nutr Biochem; Up-regulation of vitamin B1 homeostasis genes in breast cancer.; Authors - Zastre Jason A JA
human	WB	J Biol Chem; Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters.; Authors - Subrata Sabui
mouse	WB	Front Pharmacol; Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice.; Authors - Hong Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Valliammai (Verified Customer) (02-15-2023)

Results are highly reliable. Thanks for the service.

• Applications: Western Blot, Immunofluorescence, Protein purification

FH

Selvaraj (Verified Customer) (12-20-2022)

We are frequently using this antibody in our lab

• Applications: Western Blot
• Cell Tissue Type: Human intestinal cell lines