MCT4 Polyclonal antibody

• Featured Product
• KD/KO Validated

MCT4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

77

Reviews (2)

Cat no : 22787-1-AP

Synonyms

SLC16A3, MCT-4, MCT3, MCT 4

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Validation Data Gallery

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  WB analysis of HeLa using 22787-1-AP

  WB result of MCT4 antibody (22787-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT4 transfected HeLa cells.

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  WB analysis using 22787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22787-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 22787-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 22787-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 22787-1-AP (MCT4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of HepG2 cells using 22787-1-AP (MCT4 antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

• 

  IF Staining of HepG2 using 22787-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HepG2 using 22787-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human MCT4 (22787-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, rat skeletal muscle tissue, HepG2 cells, PC-3 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, human renal cell carcinoma tissue, mouse skeletal muscle tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 59 publications below
• IHC: See 10 publications below
• IF: See 10 publications below
• IP: See 1 publications below
• FC: See 3 publications below

Product Information

22787-1-AP targets MCT4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCT4 fusion protein Ag18788
• Full Name: solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
• Calculated Molecular Weight: 465 aa, 49 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC112269
• Gene Symbol: MCT4
• Gene ID (NCBI): 9123
• RRID: AB_11182479
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

Publications

Species	Application	Title
mouse	IF	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Cancer; Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer; Authors - Federica Cappellesso
human	WB	Nat Metab; Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma; Authors - Zijian Yang ; KD Validated
		Adv Sci (Weinh); SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle; Authors - Xiaowei She
mouse	IF	Nat Commun; Lactate sensing mechanisms in arterial chemoreceptor cells.; Authors - Hortensia Torres-Torrelo
mouse	WB	Adv Healthc Mater; Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature.; Authors - Zhijie Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aderonke (Verified Customer) (05-26-2023)

Immunoblotting was done to show the expression of MCT 4 in five head and neck cancer cell lines; only three of the five cell lines demonstrated this. An inhibitor was thereafter used at two differenct concentrations in UM-SCC-17A, and the expression decreased with increasing concentrations.

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: Head and neck cancer cell line

FH

Juan (Verified Customer) (07-15-2019)

We used the antibody for WB method. The band backgound is clear.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Hela cells

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