SLC15A1/PEPT1 Polyclonal antibody

SLC15A1/PEPT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21726-1-AP

Synonyms

HPECT1, HPEPT1, PEPT1, Peptide transporter 1, SLC15A1

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Validation Data Gallery

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  WB analysis using 21726-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21726-1-AP (SLC15A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21726-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21726-1-AP (SLC15A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, Caco-2 cells, HT-29 cells, MOLT-4 cells, mouse small intestine tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

21726-1-AP targets SLC15A1/PEPT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC15A1/PEPT1 fusion protein Ag16390
• Full Name: solute carrier family 15 (oligopeptide transporter), member 1
• Calculated Molecular Weight: 708 aa, 79 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC096327
• Gene Symbol: SLC15A1/PEPT1
• Gene ID (NCBI): 6564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC15A1/PEPT1 is a peptide transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides. PEPT1 mediates transepithelial transport of muramyl and N-formylated bacterial dipeptides contributing to recognition of pathogenic bacteria by the mucosal immune system. PEPT1 is primarily expressed in the small intestine (PMID: 22194420). PEPT1 is also expressed in distal colon in rodents and humans and contributes to water absorption. 71 kDa nonglycosylated PEPT1 protein can be detected (PMID: 23660505).

Publications

Species	Application	Title
human	IHC	Photodiagnosis Photodyn Ther; Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.; Authors - Kumi Ogihara