SLAMF7/CD319 Polyclonal antibody

SLAMF7/CD319 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12905-1-AP

Synonyms

SLAMF7, Membrane protein FOAP-12, FOAP-12, CS1, CRACC

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Validation Data Gallery

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  WB analysis using 12905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12905-1-AP (SLAMF7/CD319 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig spleen using 12905-1-AP

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 12905-1-AP (SLAMF7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 12905-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 12905-1-AP (SLAMF7 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 12905-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12905-1-AP (SLAMF7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12905-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12905-1-AP (SLAMF7 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: IM-9 cells, human spleen tissue, pig spleen tissue, Jurkat cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12905-1-AP targets SLAMF7/CD319 in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLAMF7/CD319 fusion protein Ag3804
• Full Name: SLAM family member 7
• Calculated Molecular Weight: 335 aa, 37 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC027867
• Gene Symbol: SLAMF7
• Gene ID (NCBI): 57823
• RRID: AB_2239192
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocyte activation molecule (SLAM) family receptors is a group of type I transmembrane receptors belonging to the immunoglobulin superfamily (PMID: 37251372). The SLAM family contains nine members that contain an extracellular segment comprising two or four Ig-like domains (V-like variable and C2-like constant), a transmembrane region and a cytoplasmic tail (PMID: 29662641). They are involved in various physiological and pathological processes, including the regulation of immunological responses and the route of viral entry. SLAMF7, also known as CS1, CRACC or CD319, is expressed on NK cells, CD8+ T lymphocytes, B lymphocytes, and mature dendritic cells (PMID: 28861320). It exerts both activating and inhibitory effects depending on the expression status of SAP or EAT-2. SLAMF7 is overexpressed in multiple myeloma and makes it a target for immunotherapy (PMID: 28861320). The native molecular weight of SLAMF7 is 37 kDa and the apparent molecular mass shifts due to glycosylation (PMID: 11698418; 36381324).

Publications

Species	Application	Title
human	WB	Int J Chron Obstruct Pulmon Dis; Analysis of Key Genes and miRNA-mRNA Networks Associated with Glucocorticoids Treatment in Chronic Obstructive Pulmonary Disease; Authors - Jian-Jun Wu