SKP1 Monoclonal antibody
SKP1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
1D5G4
Cat no : 67745-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
67745-1-Ig targets SKP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | SKP1 fusion protein Ag30709 |
Full Name | S-phase kinase-associated protein 1 |
Calculated Molecular Weight | 19 kDa |
Observed Molecular Weight | 18-20 kDa |
GenBank Accession Number | BC009839 |
Gene Symbol | SKP1 |
Gene ID (NCBI) | 6500 |
RRID | AB_2918514 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.
Protocols
Product Specific Protocols | |
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WB protocol for SKP1 antibody 67745-1-Ig | Download protocol |
IF protocol for SKP1 antibody 67745-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |