SKIV2L Polyclonal antibody

• Featured Product
• KD/KO Validated

SKIV2L Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

11

Reviews (2)

Cat no : 11462-1-AP

Synonyms

SKI2, HLP, Helicase SKI2W, EC:3.6.4.13, DDX13

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Validation Data Gallery

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  WB analysis of HEK-293 using 11462-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11462-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11462-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11462-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11462-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 11462-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11462-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11462-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11462-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11462-1-AP (SKIV2L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 11462-1-AP

  IP result of anti-SKIV2L (IP:11462-1-AP, 4ug; Detection:11462-1-AP 1:600) with HepG2 cells lysate 3200ug.

• 

  IP experiment of HepG2 using 11462-1-AP

  IP result of anti-SKIV2L (IP:11462-1-AP, 4ug; Detection:11462-1-AP 1:600) with HepG2 cells lysate 3600ug.

• 

  IHC staining of human stomach using 11462-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11462-1-AP (SKIV2L antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 11462-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11462-1-AP (SKIV2L antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, COLO 320 cells, human kidney tissue, HepG2 cells, Jurkat cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

11462-1-AP targets SKIV2L in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SKIV2L fusion protein Ag1977
• Full Name: superkiller viralicidic activity 2-like (S. cerevisiae)
• Calculated Molecular Weight: 1246 aa, 138 kDa
• Observed Molecular Weight: 138 kDa
• GenBank Accession Number: BC015758
• Gene Symbol: SKIV2L
• Gene ID (NCBI): 6499
• RRID: AB_2187472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Ski complex is a multiprotein complex required for exosome-mediated RNA surveillance, including the regulation of normal mRNA and the decay of nonfunctional mRNA. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C) [PMID:22444670]. SKIV2L , one component of the Ski complex, acts as an RNA helicase with a role in exosome recruitment or activation. It is involved in the degradation of RNAs by the exosome and may have a role in autophagy [PMID:11719186].

Publications

Species	Application	Title
	WB	Nat Immunol; The SKIV2L RNA exosome limits activation of the RIG-I-like receptors.; Authors - Sterling C Eckard
	WB	Sci Immunol; The mammalian SKIV2L RNA exosome is essential for early B cell development.; Authors - Kun Yang
human	WB	J Clin Invest; Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.; Authors - Kun Yang
human	WB	Nucleic Acids Res; Dom34 mediates targeting of exogenous RNA in the antiviral OAS/RNase L pathway.; Authors - Takuto Nogimori ; KD Validated
human	WB	Nucleic Acids Res; A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans.; Authors - Katarzyna Kalisiak
human	WB	Proc Natl Acad Sci U S A; The SKI complex is a broad-spectrum, host-directed antiviral drug target for coronaviruses, influenza, and filoviruses.; Authors - Stuart Weston ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (08-04-2024)

Antibody stains a band at ~150 kDa. However other non-specific bands are present

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

SKIV2L Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:11462-1-AP)

FH

Rachel (Verified Customer) (01-31-2019)

This antibody does produce a band at the expected size for SKIV2L, but it also lights up several lower molecular weight, non-specific bands. Additionally, it stains two different ladders that I have run as well.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: A549 cell line