SIX1 Monoclonal antibody

SIX1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig, rabbit, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D7D1

Cat no : 67741-1-Ig

Synonyms

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

---

Validation Data Gallery

• 

  WB analysis using 67741-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67741-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67741-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 67741-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

---

Tested Applications

• Positive WB detected in: L02 cells, HeLa cells, rat skeletal muscle tissue, mouse skeletal muscle tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67741-1-Ig targets SIX1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

• Tested Reactivity: Human, mouse, pig, rabbit, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIX1 fusion protein Ag28570
• Full Name: SIX homeobox 1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC008874
• Gene Symbol: SIX1
• Gene ID (NCBI): 6495
• RRID: AB_2918510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.