SIRT7 Polyclonal antibody

SIRT7 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 29729-1-AP

Synonyms

SIR2 like protein 7, SIR2L7, SIRT7

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Validation Data Gallery

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  WB analysis using 29729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29729-1-AP (SIRT7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 29729-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SIRT7 antibody (29729-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: Caco-2 cells, HeLa cells, HCT 116 cells, MCF-7 cells, rat liver cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

29729-1-AP targets SIRT7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT7 fusion protein Ag31794
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Calculated Molecular Weight: 45KD
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_016538
• Gene Symbol: SIRT7
• Gene ID (NCBI): 51547
• RRID: AB_2918342
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Publications

Species	Application	Title
human,rat	WB,IHC	Int Urol Nephrol; SIRT7 silencing by miR-152-3p confers cell apoptosis and renal functional impairment induced by renal ischaemia/reperfusion injury; Authors - Yan Wang