SIRT7 Polyclonal antibody

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  WB analysis using 12994-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12994-1-AP (SIRT7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 12994-1-AP

  IP result of anti-SIRT7 (IP:12994-1-AP, 4ug; Detection:12994-1-AP 1:1000) with PC-3 cells lysate 960 ug.

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  IHC staining of mouse liver using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

SIRT7 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12994-1-AP

Publications(26)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SIR2L7, SIR2 like protein 7, NAD-dependent protein deacylase sirtuin-7, EC:2.3.1.286, EC:2.3.1.-

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Tested Applications

• Positive WB detected in: PC-3 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below
• IHC: See 5 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

12994-1-AP targets SIRT7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT7 fusion protein Ag3656
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Calculated Molecular Weight: 400 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC017305
• Gene Symbol: SIRT7
• Gene ID (NCBI): 51547
• RRID: AB_10644276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRC8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.; Authors - Yi Fu
mouse	WB	Nat Commun; Sirt6 deficiency exacerbates podocyte injury and proteinuria through targeting Notch signaling.; Authors - Min Liu
human	WB	Aging Cell; Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells.; Authors - Weina Zhang
mouse	WB	Redox Biol; A new FGF1 variant protects against adriamycin-induced cardiotoxicity via modulating p53 activity.; Authors - Mengjie Xiao
	WB	Oxid Med Cell Longev; SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway.; Authors - Kun-Lin Chen
rat	WB	PLoS Pathog; KSHV vIL-6 promotes SIRT3-induced deacetylation of SERBP1 to inhibit ferroptosis and enhance cellular transformation by inducing lipoyltransferase 2 mRNA degradation; Authors - Jing Zhou