SIRT7 Polyclonal antibody

• Featured Product
• KD/KO Validated

SIRT7 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 12994-1-AP

Synonyms

SIR2 like protein 7, SIR2L7, SIRT7

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Validation Data Gallery

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  WB analysis of mouse liver using 12994-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12994-1-AP (SIRT7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12994-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12994-1-AP (SIRT7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 12994-1-AP

  IP Result of anti-SIRT7 (IP:12994-1-AP, 4ug; Detection:12994-1-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of rat liver using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 12994-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 12994-1-AP (SIRT7 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human kidney tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: rat liver tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

12994-1-AP targets SIRT7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIRT7 fusion protein Ag3656
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)
• Calculated molecular weight: 400 aa, 45 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC017305
• Gene symbol: SIRT7
• Gene ID (NCBI): 51547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.

Publications

Species	Application	Title
	WB	Oxid Med Cell Longev; SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway.; Authors - Kun-Lin Chen
human	IHC	Front Genet; Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer.; Authors - Xiaodan Sun
human	WB	Aging Cell; Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells.; Authors - Weina Zhang
mouse	WB	Data Brief; Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto.; Authors - Norihiro Okada
human	WB	Pharmazie; The effects of A549 and H1299 cell-derived exosomes on the proliferation and apoptosis of BEAS-2B cells.; Authors - Zhenbin Li
human	WB	Int J Clin Exp Pathol; High expression of Sirt7 served as a predictor of adverse outcome in breast cancer.; Authors - Qian Geng