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SIRT7 Polyclonal antibody
SIRT7 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12994-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, human kidney tissue |
Positive IP detected in | mouse liver tissue |
Positive IHC detected in | rat liver tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 13 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
Product Information
12994-1-AP targets SIRT7 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SIRT7 fusion protein Ag3656 |
Full Name | sirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae) |
Calculated molecular weight | 400 aa, 45 kDa |
Observed molecular weight | 45 kDa |
GenBank accession number | BC017305 |
Gene symbol | SIRT7 |
Gene ID (NCBI) | 51547 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIRT7 (NAD-dependent deacetylase sirtuin-7), also known as SIR2L7, is a member of the class IV sirtuin family and is localized to the nucleolus. Expressed throughout the body, SIRT7 associates with rDNA genes where it interacts with histones and acts as a positive regulator of RNA polymerase I (Pol I). SIRT7 is a probable NAD-dependent deacetylase whose expression is upregulated in thyroid carcinoma cells. Overexpression of SIRT7 increases Pol I-mediated transcription, thereby speeding cell growth and contributing to the development of cancer.
Protocols
Product Specific Protocols | |
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WB protocol for SIRT7 antibody 12994-1-AP | Download protocol |
IHC protocol for SIRT7 antibody 12994-1-AP | Download protocol |
IP protocol for SIRT7 antibody 12994-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oxid Med Cell Longev SIRT7 Regulates Lipopolysaccharide-Induced Inflammatory Injury by Suppressing the NF-κB Signaling Pathway. | ||
Front Genet Comprehensive Analysis of Expression and Prognostic Value of Sirtuins in Ovarian Cancer. | ||
Aging Cell Lycorine hydrochloride suppresses stress-induced premature cellular senescence by stabilizing the genome of human cells. | ||
Data Brief Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto. | ||
Pharmazie The effects of A549 and H1299 cell-derived exosomes on the proliferation and apoptosis of BEAS-2B cells. | ||
Int J Clin Exp Pathol High expression of Sirt7 served as a predictor of adverse outcome in breast cancer. |