SIRT5 Monoclonal antibody, PBS Only

SIRT5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B2

Cat no : 67257-1-PBS

Synonyms

FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5

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Validation Data Gallery

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  WB analysis of HeLa using 67257-1-Ig (same clone as 67257-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67257-1-Ig (same clone as 67257-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L02 using 67257-1-Ig (same clone as 67257-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67257-1-Ig (same clone as 67257-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67257-1-Ig (same clone as 67257-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67257-1-Ig (SIRT5 antibody) at dilution of 1:4800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67257-1-Ig (same clone as 67257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67257-1-Ig (SIRT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67257-1-PBS in a different storage buffer formulation.

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Product Information

67257-1-PBS targets SIRT5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT5 fusion protein Ag7743
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000126
• Gene Symbol: SIRT5
• Gene ID (NCBI): 23408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in . It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and contribute to the regulation of blood ammonia levels.