SIRT2 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SIRT2 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8G10

Cat no : 66410-1-PBS

Synonyms

SIR2, SIR2 like protein 2, SIR2L, SIR2L2, SIRT2

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Validation Data Gallery

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  WB analysis using 66410-1-Ig (same clone as 66410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66410-1-Ig (same clone as 66410-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66410-1-Ig (same clone as 66410-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66410-1-Ig (same clone as 66410-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66410-1-Ig (same clone as 66410-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66410-1-Ig (same clone as 66410-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig heart using 66410-1-Ig (same clone as 66410-1-PBS)

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66410-1-Ig (same clone as 66410-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66410-1-Ig (same clone as 66410-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66410-1-Ig (same clone as 66410-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66410-1-Ig (SIRT2 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66410-1-Ig (SIRT2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66410-1-Ig (same clone as 66410-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66410-1-Ig(SIRT2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66410-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66410-1-PBS targets SIRT2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT2 fusion protein Ag7756
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 2 (S. cerevisiae)
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC003547
• Gene Symbol: SIRT2
• Gene ID (NCBI): 22933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The silent information regulator(SIR2) family of genes is highly conserved from prokaryotes to eukaryotes and is involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA damage repair, and aging. SIR2 contains a 323 amino acid catalytic core domain with a NAD-binding domain and a large groove which is the likely site of catalysis. SIR2 is widely expressed and highly expressed in the heart, brain, and skeletal muscle, while it is weakly expressed in the placenta and lungs. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of the microtubule network. SIRT2 exists in various isoforms and the molecular weight of isoforms are 35 kDa, 40 kDa, and 42 kDa.