Various lysates were subjected to SDS PAGE followed by western blot with RMX00009 (SIRT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00009 (SIRT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using RMX00009
IP result of anti-SIRT1 (IP:RMX00009, 4ug; Detection:RMX00009 1:4000) with HeLa cells lysate 1200 ug.
IP result of anti-SIRT1 (IP:RMX00009, 4ug; Detection:RMX00009 1:4000) with HeLa cells lysate 1200 ug.
IHC staining of human colon cancer using RMX00009
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using RMX00009
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using RMX00009
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using RMX00009
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
IF Staining of A431 using RMX00009
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
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HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
Positive IP detected in
HeLa cells
Positive IHC detected in
human colon cancer tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:2000-1:16000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00009 targets SIRT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SIRT1, also named SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein containing one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttle between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00009 (SIRT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human colon cancer using RMX00009
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using RMX00009
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using RMX00009
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using RMX00009
IP result of anti-SIRT1 (IP:RMX00009, 4ug; Detection:RMX00009 1:4000) with HeLa cells lysate 1200 ug.
IF/ICC Figures
IF Staining of A431 using RMX00009
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
IF Staining of A431 using RMX00009
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SIRT1 Multi-Recombinant antibody
Catalog Number
RMX00009
Citations
-
Dilutions
WB : 1:2000-1:16000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:250-1:1000 IF/ICC : 1:500-1:2000
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
