Multi-rAb™ SIRT1 Multi-Recombinant antibody

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  WB analysis using RMX00009

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00009 (SIRT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using RMX00009

  IP result of anti-SIRT1 (IP:RMX00009, 4ug; Detection:RMX00009 1:4000) with HeLa cells lysate 1200 ug.

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  IHC staining of human colon cancer using RMX00009

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using RMX00009

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using RMX00009

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00009 (SIRT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using RMX00009

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:1000 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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  IF Staining of A431 using RMX00009

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIRT1 antibody (RMX00009) at dilution of 1:500 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

SIRT1 Multi-Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. RMX00009

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NAD-dependent protein deacylase sirtuin-1, NAD-dependent protein deacetylase sirtuin-1, hSIRT1, hSIR2, EC:2.3.1.286

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00009 targets SIRT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Calculated Molecular Weight: 747 aa, 82 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC012499
• Gene Symbol: SIRT1
• Gene ID (NCBI): 23411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q96EB6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRT1, also named SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein containing one deacetylase sirtuin-type domain. The 75-80 kDa SirT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SirT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin, and inner membrane. It can shuttle between the nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition, SIRT1 activation elicits resistance to oxidative stress via the regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.