SIRT1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

SIRT1 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7F6G6

Cat no : 60303-1-PBS

Synonyms

hSIR2, hSIRT1, SIR2 like protein 1, SIR2L1, SIRT1

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Validation Data Gallery

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  WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  WB analysis of Hek-293 cells using 60303-1-Ig (same clone as 60303-1-PBS)

  WB result of SIRT1antibody (60303-1-Ig, 1:500) with si-Control and si-SIRT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  WB analysis using 60303-1-Ig (same clone as 60303-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60303-1-Ig (SIRT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 60303-1-Ig (same clone as 60303-1-PBS)

  IP result of anti-SIRT1 (IP:60303-1-Ig, 5ug; Detection:60303-1-Ig 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 60303-1-Ig (same clone as 60303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60303-1-Ig (same clone as 60303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60303-1-Ig (same clone as 60303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  IHC staining of human testis using 60303-1-Ig (same clone as 60303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60303-1-Ig (SIRT1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human testis using 60303-1-Ig (same clone as 60303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 60303-1-Ig (SIRT1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60303-1-Ig (same clone as 60303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60303-1-Ig (SIRT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60303-1-Ig (same clone as 60303-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 60303-1-Ig(SIRT1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60303-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60303-1-PBS targets SIRT1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SIRT1 fusion protein Ag17677
• Full Name: sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)
• Calculated Molecular Weight: 747 aa, 82 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC012499
• Gene Symbol: SIRT1
• Gene ID (NCBI): 23411
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SIRT1, also named as SIR2L1, contains a deacetylase sirtuin-type domain and belongs to the sirtuin family. The post-translation modified SIRT1 is a 110-130 kDa protein, which contains one deacetylase sirtuin-type domain. The 75-80 kDa SIRT1 fragment was detected to lack the carboxy-terminus (PMID:21305533). SIRT1 exists a 57-61 kDa isoform. SIRT1 may be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. It can shuttles between nucleus and cytoplasm. SIRT1 regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. SIRT1 in particular initiates several signaling events relevant to cardioprotection, including: activation of endothelial nitric oxide synthase, INS receptor signaling, and autophagy. In addition SIRT1 activation elicits resistance to oxidative stress via regulation of transcription factors and co-activators such as FOXO, Hif-2a, and NF-kB. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lysine 382.