Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of SW480 using 67337-1-Ig
SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 67337-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of THP-1 using 67337-1-Ig
THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 67337-1-Ig
U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of rat cerebellum using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
rat cerebellum tissue, human liver tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67337-1-Ig targets SIRP Alpha/CD172a in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.
Protocols
Product Specific Protocols
WB protocol for SIRP Alpha/CD172a antibody 67337-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of SW480 using 67337-1-Ig
SW480 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 67337-1-Ig
HL-60 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of THP-1 using 67337-1-Ig
THP-1 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of U-251 using 67337-1-Ig
U-251 cells were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of rat cerebellum using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat cerebellum using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 67337-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SIRP Alpha/CD172a Monoclonal antibody
Catalog Number
67337-1-Ig
Citations
-
Dilutions
WB : 1:5000-1:50000 IHC : 1:2000-1:8000
Applications
WB, IHC, ELISA
Reactivity
human, mouse, rat, pig, rabbit
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
