Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody

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  FC experiment of human peripheral blood leukocytes using 98101-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP Alpha/CD172a Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human peripheral blood leukocytes using 98101-1-RR

  1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRPA Rabbit Recombinant Antibody (98101-1-RR, Clone: 240789E1) (red) or Isotype Control (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98101-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98101-1-RR against Human SIRP Alpha/CD172a were performed. The affinity constant is 3.39 nM.

SIRP Alpha/CD172a Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98101-1-RR

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

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Tested Applications

• Positive FC detected in: human peripheral blood leukocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98101-1-RR targets SIRP Alpha/CD172a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• GenBank Accession Number: NM_080792
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• RRID: AB_3672248
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P78324
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).