Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 30544-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 30544-1-AP
IP result of anti-SIPA1L3 (IP:30544-1-AP, 4ug; Detection:30544-1-AP 1:3000) with HeLa cells lysate 1320 ug.
IP result of anti-SIPA1L3 (IP:30544-1-AP, 4ug; Detection:30544-1-AP 1:3000) with HeLa cells lysate 1320 ug.
IHC staining of human ovary cancer using 30544-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30544-1-AP (SIPA1L3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30544-1-AP (SIPA1L3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 30544-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 30544-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells, HEK-293 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:1000-1:6000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
STEAP3 (Signal-induced proliferation-associated 1-like protein 3) is also named as KIAA0545 and SPAL3. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).
Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 30544-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 30544-1-AP (SIPA1L3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human ovary cancer using 30544-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30544-1-AP (SIPA1L3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 30544-1-AP
IP result of anti-SIPA1L3 (IP:30544-1-AP, 4ug; Detection:30544-1-AP 1:3000) with HeLa cells lysate 1320 ug.
IF/ICC Figures
IF Staining of HeLa using 30544-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 30544-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SIPA1L3 antibody (30544-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SIPA1L3 Polyclonal antibody
Catalog Number
30544-1-AP
Citations
1
Dilutions
WB : 1:1000-1:6000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:50-1:500 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
