SIK1 Recombinant antibody

SIK1 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240961H3

Cat no : 83897-4-RR

Synonyms

Serine/threonine-protein kinase SIK1, Salt-inducible kinase 1, salt inducible kinase 1, MSK, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 83897-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83897-4-RR (SIK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 83897-4-RR

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 83897-4-RR (SIK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 83897-4-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SIK1 antibody (83897-4-RR, Clone: 240961H3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 83897-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83897-4-RR against Human SIK1 were performed. The affinity constant is 0.382 nM.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83897-4-RR is SIK1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SIK1 fusion protein Ag28481
• Full Name: salt-inducible kinase 1
• Calculated Molecular Weight: 783 aa, 85 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC038504
• Gene Symbol: SIK1
• Gene ID (NCBI): 150094
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIK1(Salt-inducible kinase 1) is also named SIK, SNF1LK, and belongs to the CAMK Ser/Thr protein kinase family. It associates constitutively with the NK regulatory complex and is responsible for increases in its catalytic activity following small elevations in intracellular sodium concentrations(PMID:17939993). SIK1 is required for the phosphorylation of class II HDACs and expression of MEF2 target genes in myocytes(PMID:17468767). Defects in SIK1 may be associated with some cancers, such as breast cancer. Loss of SIK1 correlates with poor patient outcomes in breast cancers(PMID:19622832).