SIGMAR1 Recombinant antibody, PBS Only (Detector)

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  WB analysis using 85064-1-RR (same clone as 85064-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85064-1-RR (SIGMAR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85064-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01809-1

  Cytometric bead array standard curve of MP01809-1, SIGMAR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85064-2-PBS. Detection antibody: 85064-1-PBS. Standard: Ag7351. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 85064-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85064-1-RR against Human SIGMAR1 were performed. The affinity constant is 1.54 nM.

SIGMAR1 Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 85064-1-PBS

Clone No.242621C6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Sigma 1 type opioid receptor, OPRS1, hSigmaR1, Aging-associated gene 8 protein, AAG8

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Product Information

85064-1-PBS targets SIGMAR1 as part of a matched antibody pair:

MP01809-1: 85064-2-PBS capture and 85064-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SIGMAR1 fusion protein Ag7351
• Full Name: sigma non-opioid intracellular receptor 1
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC004899
• Gene Symbol: SIGMAR1
• Gene ID (NCBI): 10280
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99720
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 25 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.