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SIGLEC6 Polyclonal antibody

SIGLEC6 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 13454-1-AP

Synonyms

CD327, CD33 antigen like 1, CD33L, CD33L1, CDw327, OB BP1, OBBP1, Obesity binding protein 1, SIGLEC 6, SIGLEC6

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using SIGLEC6 Polyclonal antibody (13454-1-AP)

    IHC staining of human placenta using 13454-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (SIGLEC6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SIGLEC6 Polyclonal antibody (13454-1-AP)

    IHC staining of human placenta using 13454-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13454-1-AP (SIGLEC6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13454-1-AP targets SIGLEC6 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SIGLEC6 fusion protein Ag4281
Full Name sialic acid binding Ig-like lectin 6
Calculated Molecular Weight 426 aa, 47 kDa
GenBank Accession NumberBC035359
Gene Symbol SIGLEC6
Gene ID (NCBI) 946
RRIDAB_2189287
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SIGLEC6 antibody 13454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Emma (Verified Customer) (02-22-2022)

Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:25
  • Cell Tissue Type: Prostate