SIAE Polyclonal antibody

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  WB analysis using 14566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14566-1-AP (SIAE antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SIAE Polyclonal Antibody for WB, ELISA

Cat No. 14566-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat heart tissue, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14566-1-AP targets SIAE in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SIAE fusion protein Ag6143
• Full Name: sialic acid acetylesterase
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 62-70 kDa
• GenBank Accession Number: BC068450
• Gene Symbol: SIAE
• Gene ID (NCBI): 54414
• RRID: AB_3669189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HAT2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sialic acids as terminal sugars of N- and O-glycosylated macromolecules are decisive for the functions of glycoconjugates both in soluble form and integrated into cell membranes .The two enzymes responsible for acetylation and de-acetylation have been described as sialate O-acetyltransferase (SOAT) and sialate O-acetylesterase (SIAE), respectively. SIAE deacetylates α(2-6)-linked sialic acid on N-linked glycans of BCR, allowing the interaction with CD22, that functions in vivo as an inhibitor of BCR signaling. (PMID: 26022516)