SI-CLP,CHID1 Polyclonal antibody

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  WB analysis using 19900-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19900-1-AP (SI-CLP,CHID1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

SI-CLP,CHID1 Polyclonal Antibody for WB, ELISA

Cat No. 19900-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CHID1, SB139, PSEC0104, GL008, Chitinase domain-containing protein 1

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Tested Applications

• Positive WB detected in: HEK-293 cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19900-1-AP targets SI-CLP,CHID1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SI-CLP,CHID1 fusion protein Ag13806
• Full Name: chitinase domain containing 1
• Calculated Molecular Weight: 393 aa, 45 kDa
• Observed Molecular Weight: 42-48 kDa
• GenBank Accession Number: BC000001
• Gene Symbol: CHID1
• Gene ID (NCBI): 66005
• RRID: AB_3085591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BWS9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHID1 (chitinase domain containing protein 1) is a member of the Glyco_18 domain-containing proteins family, expressed by type 2 macrophages upon their stimulation with interleukin (IL)-4 and glucocorticoids. Expression of CHID1 is documented for TAMs in various tumors, but its role in tumor development remains unclear. (PMID: 33466316)