SHPK Recombinant antibody, PBS Only

SHPK Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230115A4

Cat no : 82875-1-PBS

Synonyms

SHK, Sedoheptulokinase, EC:2.7.1.14, CARKL, Carbohydrate kinase-like protein

---

Validation Data Gallery

• 

  IF Staining of U2OS using 82875-1-RR (same clone as 82875-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using SHPK antibody (82875-1-RR, Clone: 230115A4 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U-2 OS using 82875-1-RR (same clone as 82875-1-PBS)

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug Anti-Human SHPK (82875-1-RR, Clone:230115A4) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82875-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82875-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82875-1-RR against Human SHPK were performed. The affinity constant is 18.1 pM.

---

Product Information

82875-1-PBS targets SHPK in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SHPK fusion protein Ag33893
• Full Name: sedoheptulokinase
• Calculated Molecular Weight: 478 aa, 52 kDa
• GenBank Accession Number: BC020543
• Gene Symbol: SHPK
• Gene ID (NCBI): 23729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).