SHMT2 Monoclonal antibody, PBS Only

SHMT2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A2H9

Cat no : 67980-1-PBS

Synonyms

GLYA, Serine methylase, SHMT, SHMT2

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Validation Data Gallery

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  WB analysis using 67980-1-Ig (same clone as 67980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67980-1-Ig (same clone as 67980-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67980-1-Ig (SHMT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67980-1-Ig (same clone as 67980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67980-1-Ig (SHMT2 antibody) at dilution of 1:1300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67980-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67980-1-PBS targets SHMT2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHMT2 fusion protein Ag1584
• Full Name: serine hydroxymethyltransferase 2 (mitochondrial)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 52-55 kDa
• GenBank Accession Number: BC011911
• Gene Symbol: SHMT2
• Gene ID (NCBI): 6472
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.