Tested Applications
| Positive WB detected in | HeLa cells, rat kidney tissue, HepG2 cells, LNCaP cells, PC-3 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue, human pancreas cancer tissue, human ovary tumor tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse kidney tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20697-1-AP targets SHH in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, mink |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | sonic hedgehog homolog (Drosophila) |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 45-51 kDa |
| GenBank Accession Number | NM_000193 |
| Gene Symbol | SHH |
| Gene ID (NCBI) | 6469 |
| RRID | AB_10694828 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15465 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sonic hedgehog (SHH) is a member of the Hedgehog (Hh) family of proteins that control cell growth, survival,
and fate.
What is the molecular weight of SHH?
The molecular weight of SHH is approximately 45 kDa, but it is cleaved autocatalytically to generate a 20 kDa
N-terminal fragment and a 25 kDa C-terminal fragment responsible for hedgehog biological activity and the
auto-processing machinery respectively (PMID: 10753901).
What is the tissue specificity of SHH?
SHH is the most broadly expressed mammalian Hh signaling molecule. During early vertebrate development,
SHH is expressed in midline tissues such as the node, notochord, and floor plate to control the left-right and
dorso-ventral patterning of the embryo (PMID: 12729563). SHH is expressed in fetal intestine, liver, kidney,
and lung tissue, but is not expressed in adult tissues.
What is the cellular localization of SHH?
SHH is localized to the cell membrane. The N-terminal fragment is associated with lipid rafts at the cell surface,
whereas the C-terminal fragment diffuses from the cell.
What is the role of SHH in the Sonic hedgehog signaling pathway?
This pathway is one of the major trafficking networks that regulate key events during developmental growth
and patterning (PMID: 19368798). Loss of SHH signaling and regulation has been implicated in birth defects,
tissue regeneration, stem cell renewal, and cancer growth (PMID: 15549094). The regulatory action of the SHH
pathway is linked to the secretion, uptake, and translocation of the SHH protein; a ligand precursor, which is
implicated in the regulation of the central nervous system polarity and neural patterning (PMID: 11130178,
11801325).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SHH antibody 20697-1-AP | Download protocol |
| IHC protocol for SHH antibody 20697-1-AP | Download protocol |
| IP protocol for SHH antibody 20697-1-AP | Download protocol |
| WB protocol for SHH antibody 20697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
SHH Polyclonal antibody (Cat# 20697-1-AP) has accumulated 105 citations across journals including Nature Communications, Journal of Clinical Investigation, Cell Reports, Oncogene, EMBO Molecular Medicine, Theranostics, Acta Pharmacologica Sinica, and iScience, among others. Cited research primarily focuses on Hedgehog signaling pathway regulation in various cancers (colorectal, gastric, lung, bladder, glioma), stem cell biology, neural regeneration, fibrosis, developmental biology, and epithelial-mesenchymal transition.
| Species | Application | Title |
|---|---|---|
Cell Host Microbe BCAA-producing Clostridium symbiosum promotes colorectal tumorigenesis through the modulation of host cholesterol metabolism | ||
Sci Bull (Beijing) Restoring sweat gland function in mice using regenerative sweat gland cells derived from chemically reprogrammed human epidermal keratinocytes | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
J Nanobiotechnology NaHS@Cy5@MS@SP nanoparticles improve rheumatoid arthritis by inactivating the Hedgehog signaling pathway through sustained and targeted release of H2S into the synovium | ||
Theranostics Single-cell Transcriptome Profiling reveals Dermal and Epithelial cell fate decisions during Embryonic Hair Follicle Development. | ||
J Clin Invest Superenhancer-activation of KLHDC8A drives glioma ciliation and hedgehog signaling |
Reviews
What customers say
The single review for 20697-1-AP (4/5 stars) was posted by Quentin Brassart on March 28, 2024. Using a 1:200 dilution with lot 00105598 on neural culture, the reviewer reported that the antibody performed perfectly for Western Blot but did not work well for Immunofluorescence on cell culture. Overall, the antibody is considered reliable for WB applications but may have limitations in IF on cultured cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Quentin (Verified Customer) (03-28-2024) | It has worked perfectly for Western-Blot but not in Immunofluorescence on cell culture.
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