SHCBP1 Monoclonal antibody

SHCBP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E12

Cat no : 68647-1-Ig

Synonyms

SHC SH2 domain-binding protein 1, PAL

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Validation Data Gallery

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  WB analysis using 68647-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68647-1-Ig (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 68647-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 68647-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 68647-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 68647-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68647-1-Ig (SHCBP1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 68647-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SHCBP1 antibody (68647-1-Ig, Clone: 1B2E12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Tested Applications

• Positive WB detected in: PC-3 cells, U2OS cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68647-1-Ig targets SHCBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHCBP1 fusion protein Ag17517
• Full Name: SHC SH2-domain binding protein 1
• Calculated Molecular Weight: 672 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC030699
• Gene Symbol: SHCBP1
• Gene ID (NCBI): 79801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.