SHCBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

SHCBP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 12672-1-AP

Synonyms

PAL, SHCBP1

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Validation Data Gallery

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  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 12672-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 12672-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12672-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12672-1-AP

  IP result of anti-SHCBP1 (IP:12672-1-AP, 4ug; Detection:12672-1-AP 1:500) with Jurkat cells lysate 2400ug.

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  IHC staining of human liver cancer using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, Sp2/0 cells, Neuro-2a cells, HepG2 cells, mouse thymus tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human liver cancer tissue, human tonsillitis tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12672-1-AP targets SHCBP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHCBP1 fusion protein Ag3355
• Full Name: SHC SH2-domain binding protein 1
• Calculated Molecular Weight: 672 aa, 76 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC030699
• Gene Symbol: SHCBP1
• Gene ID (NCBI): 79801
• RRID: AB_10639526
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

Publications

Species	Application	Title
mouse	WB	Nat Neurosci; Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus.; Authors - Phan Q Duy
human	WB,IF,IP	Genomics; SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.; Authors - Can Yang ; KD Validated
human	WB,IHC	Am J Cancer Res; SHCBP1 regulates STAT3/c-Myc signaling activation to promote tumor progression in penile cancer.; Authors - Miao Mo ; KD Validated
human	WB	J Virol; Measles virus non-structural C protein modulates viral RNA polymerase activity by interacting with a host protein SHCBP1.; Authors - Ito Minako M ; KD Validated
mouse	WB	Mol Carcinog; Knockout of Shcbp1 sensitizes immunotherapy by regulating α-SMA positive cancer-associated fibroblasts; Authors - Qianlin Gu ; KO Validated
human	IF,IHC, WB	J Exp Clin Cancer Res; SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis.; Authors - Changliang Peng ; KD Validated