SHC1 Monoclonal antibody

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  WB analysis using 67194-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of hTERT-RPE1 using 67194-2-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67194-2-Ig (SHC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

SHC1 Monoclonal Antibody for WB, ELISA

Cat No. 67194-2-Ig

Clone No.1A5B1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: TT cells, hTERT-RPE1 cells, PANC-1 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67194-2-Ig targets SHC1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SHC1 fusion protein Ag25173
• Full Name: SHC (Src homology 2 domain containing) transforming protein 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_183001
• Gene Symbol: SHC1
• Gene ID (NCBI): 6464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29353
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.