SHBG Polyclonal antibody

SHBG Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 18200-1-AP

Synonyms

ABP, Androgen binding protein, SBP, sex hormone binding globulin, Sex steroid binding protein, SHBG, TEBG

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Validation Data Gallery

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  WB analysis using 18200-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 18200-1-AP (SHBG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 18200-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18200-1-AP (SHBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 18200-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18200-1-AP (SHBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human plasma
• Positive IHC detected in: mouse testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

18200-1-AP targets SHBG in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHBG fusion protein Ag12683
• Full Name: sex hormone-binding globulin
• Calculated Molecular Weight: 402 aa, 44 kDa
• Observed Molecular Weight: 42-44 kDa
• GenBank Accession Number: BC101785
• Gene Symbol: SHBG
• Gene ID (NCBI): 6462
• RRID: AB_2878517
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

Publications

Species	Application	Title
goat	WB	Mol Cell Endocrinol; FSH inhibits autophagy and lysosomal biogenesis to regulate protein degradation in cultured goat Sertoli cells.; Authors - Huaming Xi
goat	WB	Biol Reprod; Changes in histology, protein expression, and autophagy in dairy goat testes during non-breeding season.; Authors - Huaming Xi
	WB	Theriogenology; FSH-inhibited autophagy protects against oxidative stress in goat Sertoli cells through p62-Nrf2 pathway; Authors - Huaming Xi