SHANK1 Polyclonal antibody

SHANK1 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 55059-1-AP

Synonyms

SHANK1, SPANK 1, SSTR interacting protein, SSTRIP, synamon

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Validation Data Gallery

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  WB analysis using 55059-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55059-1-AP (SHANK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 55059-1-AP

  IP result of anti-SHANK1 (IP:55059-1-AP, 4ug; Detection:55059-1-AP 1:400) with HEK-293T cells lysate 1155 ug.

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  IF Staining of mouse eye using 55059-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse eye using 55059-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using SHANK1 antibody (55059-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, Neuro-2a cells
• Positive IP detected in: HEK-293T cells
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

55059-1-AP targets SHANK1 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SH3 and multiple ankyrin repeat domains 1
• Calculated Molecular Weight: 225 kDa
• Observed Molecular Weight: 159-225 kDa
• GenBank Accession Number: NM_016148
• Gene Symbol: SHANK1
• Gene ID (NCBI): 50944
• RRID: AB_10863130
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SHANK1 belongs to the SHANK family. It seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. SHANK1 may play a role in the structural and functional organization of the dendritic spine and synaptic junction. The antibody is specific to SHANK1.

Publications

Species	Application	Title
mouse	WB	Cell Res; METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation.; Authors - Zeyu Zhang
rat	WB	Neural Regen Res; Protective effects of Bushen Tiansui decoction on hippocampal synapses in a rat model of Alzheimer's disease.; Authors - Shan Hui