POSH Polyclonal antibody

• Featured Product
• KD/KO Validated

POSH Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 14649-1-AP

Synonyms

KIAA1494, Plenty of SH3s, POSH, Protein POSH, RING finger protein 142, RNF142, SH3 multiple domains protein 2, SH3MD2, SH3RF1

---

Validation Data Gallery

• 

  WB analysis of mouse brain using 14649-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14649-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 14649-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14649-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14649-1-AP (POSH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14649-1-AP

  IP result of anti-POSH (IP:14649-1-AP, 4ug; Detection:14649-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14649-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14649-1-AP (POSH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, DU 145 cells, HEK-293 cells, human brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

14649-1-AP targets POSH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, drosophila
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POSH fusion protein Ag6243
• Full Name: SH3 domain containing ring finger 1
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 93-100 kDa
• GenBank Accession Number: BC053671
• Gene Symbol: SH3RF1/POSH
• Gene ID (NCBI): 57630
• RRID: AB_2187290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.

Publications

Species	Application	Title
drosophila	IF	J Cell Biol; Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia.; Authors - Ryan J H West
mouse	WB	Int J Mol Sci; Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions.; Authors - Sunil Goodwani
mouse	IF	Hum Mol Genet; The Pro-Apoptotic JNK Scaffold POSH/SH3RF1 Mediates CHMP2BIntron5-Associated Toxicity in Animal Models of Frontotemporal Dementia.; Authors - Ryan J H West
human,mouse	WB,IF,IP	Expert Opin Ther Targets; POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.; Authors - Xinshi Wang ; KD Validated
human,mouse	WB,IP	Cell Rep; POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function.; Authors - Minghui Yao
human	WB	Int J Biol Macromol; Snakehead vesiculovirus hijacks SH3RF1 for replication via mediating K63-linked ubiquitination at K264 of the phosphoprotein; Authors - Xiangmou Qin