SH3PXD2A Polyclonal antibody

• Featured Product
• KD/KO Validated

SH3PXD2A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 18976-1-AP

Synonyms

Adapter protein TKS5, FISH, KIAA0418, SH3 and PX domains 2A, SH3 multiple domains protein 1, SH3MD1, SH3PXD2A, TKS5

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Validation Data Gallery

• 

  WB analysis of HeLa using 18976-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 18976-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18976-1-AP (SH3PXD2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18976-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18976-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 18976-1-AP

  Immunofluorescent analysis of HepG2 cells using 18976-1-AP (SH3PXD2A antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

18976-1-AP targets SH3PXD2A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SH3 and PX domains 2A
• Calculated Molecular Weight: 125 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_014631
• Gene Symbol: SH3PXD2A
• Gene ID (NCBI): 9644
• RRID: AB_10598319
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3PXD2A is also named as FISH, KIAA0418, SH3MD1, TKS5. SH3PXD2A contains an amino-terminal PX domain followed by five SH3 domains. It is a cytoplasmic protein in normal fibroblasts (PMID: 19464300). The p140 and p130 forms of SH3PXD2A may be generated by other splice variations. The complexity of SH3PXD2A isoforms is also evident in different cell types. For example in human platelets a single band of 150 kDa was detected, whereas all three forms were found in human fibroblasts and vascular smooth muscle cells (PMID: 9687503). This antibody is specific to SH3PXD2A.

Publications

Species	Application	Title
human	WB	Nat Commun; Cytoplasmic LIF reprograms invasive mode to enhance NPC dissemination through modulating YAP1-FAK/PXN signaling.; Authors - Shu-Chen Liu
mouse	IF	Circ. Res.; Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.; Authors - Dong Ma
human	IF	Adv Healthc Mater; Fiber Microarchitecture in Interpenetrating Collagen-Alginate Hydrogel with Tunable Mechanical Plasticity Regulates Tumor Cell Migration; Authors - Zhao Wei
		Theranostics; IKKε phosphorylates kindlin-2 to induce invadopodia formation and promote colorectal cancer metastasis.; Authors - Ge Liu
human	WB	Oncotarget; The role of Tks adaptor proteins in invadopodia formation, growth and metastasis of melanoma.; Authors - Shinji Iizuka ; KD Validated
human	WB,IHC,IF	J Oncol; Tumour: Fibroblast Interactions Promote Invadopodia-Mediated Migration and Invasion in Oral Squamous Cell Carcinoma; Authors - Koroku Kato ; KD Validated

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