HeLa cells were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15897-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15897-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15897-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
human testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 15897-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
rat lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 15897-1-AP
IP result of anti-SH3GLB2 (IP:15897-1-AP, 4ug; Detection:15897-1-AP 1:1000) with HeLa cells lysate 2600ug.
IP result of anti-SH3GLB2 (IP:15897-1-AP, 4ug; Detection:15897-1-AP 1:1000) with HeLa cells lysate 2600ug.
IHC staining of human placenta using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 15897-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3GLB2 antibody (15897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3GLB2 antibody (15897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HeLa cells, human testis tissue, mouse lung tissue, mouse testis tissue, rat lung tissue
Positive IP detected in
HeLa cells
Positive IHC detected in
mouse brain tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:2000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
SH3GLB2 is an endophilin-related protein family with 395-amino acid protein and contains an N-terminal domain, a central coiled-coil region, and a C-terminal SH3 domain. SH3GLB2 is expressed in many tissues, including skeletal muscle, adipocytes, brain, lung, colon, and mammary gland. SH3GLB2 was expressed in the cytoplasm of transfected HeLa cells and was excluded from nuclei(PMID: 11161816).
HeLa cells were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 15897-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 15897-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 15897-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of rat lung using 15897-1-AP
rat lung tissue were subjected to SDS PAGE followed by western blot with 15897-1-AP (SH3GLB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human placenta using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 15897-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15897-1-AP (SH3GLB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of HeLa using 15897-1-AP
IP result of anti-SH3GLB2 (IP:15897-1-AP, 4ug; Detection:15897-1-AP 1:1000) with HeLa cells lysate 2600ug.
IF/ICC Figures
IF Staining of HeLa using 15897-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SH3GLB2 antibody (15897-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
SH3GLB2 Polyclonal antibody
Catalog Number
15897-1-AP
Citations
2
Dilutions
WB : 1:500-1:2000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
