SH3GL1 Polyclonal antibody

SH3GL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27014-1-AP

Synonyms

CNSA1, EEN, EEN fusion partner of MLL, Endophilin 2, Endophilin A2, SH3 domain GRB2 like 1, SH3 domain protein 2B, SH3D2B, SH3GL1, SH3P8

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Validation Data Gallery

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  WB analysis of rat brain using 27014-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 27014-1-AP (SH3GL1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 27014-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 27014-1-AP (SH3GL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 27014-1-AP is SH3GL1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3GL1 fusion protein Ag25550
• Full Name: SH3-domain GRB2-like 1
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41-43 kDa
• GenBank Accession Number: BC001270
• Gene Symbol: SH3GL1
• Gene ID (NCBI): 6455
• RRID: AB_2880722
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	J Cell Sci; Tubular microdomains of Rab7-positive endosomes retrieve TrkA, a mechanism disrupted in Charcot-Marie-Tooth disease 2B.; Authors - Ronja Markworth
mouse	WB	FASEB J; Endophilin A2 protects against renal fibrosis by targeting TGF-β/Smad signaling; Authors - Xiaoyi Mai