SH3BP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

SH3BP5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11127-2-AP

Synonyms

SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5

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Validation Data Gallery

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  WB analysis of Raji using 11127-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11127-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human ovary tumor using 11127-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Tested Applications

• Positive WB detected in: Raji cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:500
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below

Product Information

11127-2-AP targets SH3BP5 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3BP5 fusion protein Ag1592
• Full Name: SH3-domain binding protein 5 (BTK-associated)
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 32 kDa, 50-83 kDa
• GenBank Accession Number: BC010123
• Gene Symbol: SH3BP5
• Gene ID (NCBI): 9467
• RRID: AB_2189373
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.

Publications

Species	Application	Title
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
mouse	WB	J Hepatol; Sab (Sh3bp5) dependence of JNK mediated inhibition of mitochondrial respiration in palmitic acid induced hepatocyte lipotoxicity.; Authors - Sanda Win ; KD Validated
mouse	WB	Cell Death Dis; JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death.; Authors - S Win ; KD Validated
human,mouse	WB	Front Pharmacol; Scoparone Improves Nonalcoholic Steatohepatitis Through Alleviating JNK/Sab Signaling Pathway-Mediated Mitochondrial Dysfunction.; Authors - Yuwei Jiang ; KD Validated
human	WB	Biomolecules; Elevated SH3BP5 Correlates with Poor Outcome and Contributes to the Growth of Acute Myeloid Leukemia Cells.; Authors - Minjing Li ; KD Validated
mouse	WB	Biomed Pharmacother; Fluorofenidone protects against acute liver failure in mice by regulating MKK4/JNK pathway; Authors - Lei Gu