SH3BP4 Monoclonal antibody

SH3BP4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2G4H3

Cat no : 68045-1-Ig

Synonyms

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP

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Validation Data Gallery

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  WB analysis using 68045-1-Ig

  Various cell lysates were subjected to SDS PAGE followed by western blot with 68045-1-Ig (SH3BP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

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Tested Applications

• Positive WB detected in: Y79 cells, U2OS cells, A549 cells, HeLa cells, HepG2 cells, hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68045-1-Ig targets SH3BP4 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SH3BP4 fusion protein Ag31238
• Full Name: SH3-domain binding protein 4
• Calculated Molecular Weight: 963 aa, 108 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC057396
• Gene Symbol: SH3BP4
• Gene ID (NCBI): 23677
• RRID: AB_2918787
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.