SH3BP4 Polyclonal antibody

• Featured Product
• KD/KO Validated

SH3BP4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17691-1-AP

Synonyms

BOG25, EH binding protein 10, EHB10, SH3 domain binding protein 4, SH3BP4, TTP

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Validation Data Gallery

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  WB analysis using 17691-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17691-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17691-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17691-1-AP (SH3BP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 17691-1-AP

  IP result of anti-SH3BP4 (IP:17691-1-AP, 4ug; Detection:17691-1-AP 1:300) with mouse lung tissue lysate 3600ug.

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  IF Staining of HeLa using 17691-1-AP

  Immunofluorescent analysis of HeLa cells using 17691-1-AP (SH3BP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17691-1-AP targets SH3BP4 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SH3BP4 fusion protein Ag11847
• Full Name: SH3-domain binding protein 4
• Calculated Molecular Weight: 963 aa, 108 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC057396
• Gene Symbol: SH3BP4
• Gene ID (NCBI): 23677
• RRID: AB_2189364
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Exp Mol Med; SH3BP4, a novel pigmentation gene, is inversely regulated by miR-125b and MITF.; Authors - Kyu-Han Kim ; KD Validated
pig	WB	J Appl Physiol (1985); Differential regulation of mTORC1 activation by leucine and β-hydroxy-β-methylbutyrate in skeletal muscle of neonatal pigs.; Authors - Agus Suryawan
		Nat Commun; Spatially resolved phosphoproteomics reveals fibroblast growth factor receptor recycling-driven regulation of autophagy and survival; Authors - Joanne Watson